Rationale

 

Bacterial endospores, of the type by Bacillus and Clostridium species, are known to be highly resistant to various forms of radiation and other physical and chemical agents. High-intensity ultraviolet light, however, was suspected to overcome such resistance and to kill spores efficiently. To test this hypothesis, I irradiated samples of Bacillus subtilis spores (in collaboration with Roger Williams, Xenon Corporation) and measured the loss of viability as a function of UV dose and position with respect to the axis of the lamp.

 

Protocol

DOWNLOAD TO LEARN MORE